解剖学报

›› 2012, Vol. 43 ›› Issue (4): 451-457.doi: 10.3969/j.issn.0529-1356.2012.04.004

• 神经生物学 • 上一篇    下一篇

4-苯基丁酸钠盐通过抑制内质网应激反应改善大鼠创伤性脑损伤

王焕1; 崔建忠2; 田艳霞1; 李冉1; 洪铭岩2; 王凯杰2; 高俊玲1*   

  1. 1.河北联合大学基础医学院，河北省煤矿卫生与安全实验室，河北 唐山 063000; 2.唐山市工人医院神经外科，河北 唐山 063000
  • 收稿日期:2011-12-15 修回日期:2012-02-20 出版日期:2012-08-06
  • 通讯作者: 高俊玲

Sodium 4-phenylbutyrate ameliorates traumatic brain injury in rats by reducing endoplasmic reticulum stress

  1. 1.School of Basic Medical Sciences, Hebei United University, Hebei Province Key Laboratory of Occupational Health and Safety for Coal Industry, Hebei Tangshan 063000,China; 2.Department of Neurosurgery, the Tangshan Gongren Hospital, Hebei Tangshan 063000,China
  • Received:2011-12-15 Revised:2012-02-20 Online:2012-08-06
  • Contact: GAO Jun-ling

关键词: 脑创伤, 内质网应激, 4-苯基丁酸钠盐, 葡萄糖调控蛋白78, 胰腺内质网激酶, C/EBP同源蛋白, 免疫组织化学, 大鼠

Abstract: Objective To investigate the expression of the endoplasmic reticulum stress-associated proteins glucose regulated protein 78 (GRP78), phosphorylation pancreatic ER kinase (p-PERK) and C/EBP homologous protein (CHOP) in rats after traumatic brain injury (TBI), and the mechanism of sodium 4-phenylbutyrate (4-PBA) on neuronal injury by reducing endoplasmic reticulum stress after TBI. Methods A total of 114 male Sprague-Dawley rats were randomly divided into 3 groups: the sham, TBI, TBI plus 4-PBA groups. A rat model of diffuse brain injury was established according to Marmarou’s falling model. The rats in 4-PBA group were treated with 4-PBA (120 mg/kg) i.p. immediately after TBI, once a day for 3 days. At 3, 6, 12, 24, 48 and 72 hours after injury, the brain tissue samples were prepared for examination of brain pathological changes, and determination of the expression of GRP78, p-PERK and CHOP, by means of immunohistochemistry and Western blotting. Behavioral tests were performed at 24, 48 and 72 hours after injury. Results Compared with the sham group, GRP78, p-PERK and CHOP were significantly increased in the TBI group. GRP78 began to increase at 3 hours after injury, peaked at the 12 hours, and then gradually returned to the basic level. P-PERK peaked at 12 hours, whereas, CHOP peaked at 24 hours,and then reduced at 48-72 hours,but still was higher than the basic level. At the corresponding times, compared with the TBI group, the expression of GRP78, p-PERK and CHOP decreased remarkably in the 4-PBA group（EM>P/EM> <0.05）. The scores of behavioral test were significantly increased in 4-PBA group,compared with TBI group（EM>P/EM> <0.05）. Conclusion Endoplasmic reticulum stress is started after traumatic brain injury in rats. 4-PBA has a protective effect on TBI, which ma

Key words: Traumatic brain injury, Endoplasmic reticulum stress, Sodium 4-phenylbutyrate, Glucose regulated protein 78, Pancreatic ER kinase, C/EBP homologous protein, Immunohistochemistry, Rat

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